This service runs in batches of at least 24 samples — that is how the lab schedules it, so smaller runs are not offered.
Volume pricing
24–47 samples249,00 € / sample
48–95 samples149,00 € / sample
96+ samples99,00 € / sample
Genomic DNA requirements (CytoScan HT-CMA)
The assay uses 100 ng of gDNA in 20 µL; please send at least 200 ng at 20 ng/µL or above.
The assay consumes 100 ng of genomic DNA in a total volume of 20 µL for the whole-genome amplification step (Thermo Fisher, MAN0018214).
Please send at least 200 ng per sample at 20 ng/µL or above. The surplus covers intake QC and allows a repeat run without resubmission.
Dissolved in nuclease-free water or reduced-EDTA TE buffer (10 mM Tris-HCl pH 8.0, 0.1 mM EDTA). High EDTA and residual salts inhibit the enzymatic steps.
Concentration determined fluorometrically (Qubit or PicoGreen). DNA must be double-stranded -- no boiling or strong denaturants, as single-stranded DNA cannot be quantified correctly.
Not degraded: on a 1% agarose gel roughly 90% of the material should run above 10 kb.
Free of polymerase inhibitors, in particular heme from blood and chelating agents. OD260/280 between 1.8 and 2.0, OD260/230 above 1.5; please clean up samples below these values before shipping.
Ship chilled or at ambient temperature; dry ice is not required for purified DNA.