Molecular Karyotyping

CytoScan HD incl. DNA Isolation

Name: CytoScan HD incl. DNA Isolation
Brand: Atlas Biolabs
SKU: karyotyping-hd-dna-iso
Price: 590.00 EUR
Availability: InStock

CytoScan HD molecular karyotyping as a full service for iPSC and other cell lines, including DNA isolation from submitted cell pellets and quality control.

Direct

About this service

PlatformAffymetrix GeneChip (CytoScan)

Data provided

Original signal intensities (CEL file), experiment file (ARR file) and QC report.
CYCHP files (generated by Chromosome Analysis Suite).
Standard report with significant segments: minimum genomic segment size (default 100 Kb); minimum marker count (default 10); minimum LOH size (default 5 Mb).
Upon request further bioinformatic analyses are available (additional cost will apply).

Workflow

DNA isolation from the submitted cell pellets and quality/quantity control by gel electrophoresis and Nanodrop.
Sample preparation including restriction digestion, adapter ligation, PCR, fragmentation and labeling. During processing, success of the PCR and fragmentation is assessed by gel electrophoresis (QC gel 1 and 2). A positive control per batch ensures process quality; a negative control excludes DNA contamination.
Hybridization, dyeing, washing, and scanning of the appropriate ThermoFisher/Affymetrix microarray.
Generation of CYCHP files using the Affymetrix software Chromosome Analysis Suite.

Service-specific terms

The stated price is an all-inclusive per-sample price covering DNA isolation and quality control.
The stated turnaround begins upon sample acceptance and successful intake QC, not upon sample arrival.
If the extracted DNA does not meet the sample requirements for the service, only the cost of DNA isolation will be charged. Further agreements and additional costs may apply.
Additional costs for data media (e.g. hard drives or usb drives) and delivery are not included and will be charged accordingly.

Ship samples to

Atlas Life Sciences GmbH
c/o Atlas Biolabs
Aroser Allee 68, 13407 Berlin, Germany

Configure

Your order

Sample count

Between 1 and 500 samples

Volume pricing

1–29 samples670,00 € / sample
30–59 samples640,00 € / sample
60–119 samples620,00 € / sample
120+ samples590,00 € / sample

Sample type *

Frozen cell pellet requirements

2 to 5 million PBS-washed cells per sample, one Safe-Lock tube, shipped on dry ice.

2 to 5 × 10^6 PBS-washed, pelleted cells per sample in one Safe-Lock tube.
Ship on sufficient dry ice, tubes packed in padded boxes.
Isolation and intake QC are on us; if the extracted DNA does not meet the requirements, only the isolation is charged.

DNA isolation included

This service isolates genomic DNA from your frozen cell pellet — no separate extraction step or cost. The isolation is already part of the price; the lab measures DNA quantity and quality after receiving your samples.

QC handling *

Stop and contact me before proceedingI will send a replacement pelletIf the extracted DNA does not meet the sample requirements, only the cost of DNA isolation is charged.

Use case (optional)

Notes (optional)

Catalogue