Microbiome

16S Amplicon Sequencing (V4)

Name: 16S Amplicon Sequencing (V4)
Brand: Atlas Biolabs
SKU: microbiome-16s-v4
Price: 83.00 EUR
Availability: InStock

16S rRNA V4 amplicon sequencing for microbial community profiling with high throughput. The price covers raw data only (FASTQ); bioinformatic analysis (taxonomic/functional profiling, QC) is available at additional cost and is not bundled.

Direct

Data provided

At least 50,000 read pairs per sample.
Demultiplexed FASTQ files per sample.
Run and library QC report.

Workflow

Incoming quality control of each DNA sample (concentration and purity).
Amplification of the 16S rRNA V4 region (515F/806R) and indexing for sequencing.
Pooling, library QC and sequencing in paired-end 2x300 bp mode.
Demultiplexing and delivery of per-sample FASTQ files.

Ship samples to

Atlas Life Sciences GmbH
c/o Atlas Biolabs
Aroser Allee 68, 13407 Berlin, Germany

Configure

Your order

Sample count

Between 1 and 1000 samples

Sample type *

Genomic DNA Requirements

High-quality gDNA; no additional extraction required.

High-quality genomic DNA in 0.1 mM EDTA / 10 mM Tris-HCl, pH 8.0; DNase-free RNase treatment recommended
50 ng/µL (PicoGreen or Qubit)
≥25 µL per sample (≥1.25 µg total)
Double-stranded, free of PCR inhibitors; not highly degraded
Deliver each DNA sample in a single 1.5 mL safe-lock tube
Ship on sufficient dry ice

QC handling *

Proceed at risk (free)Contact me first (free)Replace sample (25 € fee each)

Use case (optional)

Notes (optional)

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