ITS2 amplicon sequencing for fungal and yeast profiling, 2 x 300 bp, approx. 50,000 read pairs per sample; can be run from the same DNA extract as 16S.
Direct
Data provided
At least 50,000 read pairs per sample.
Demultiplexed FASTQ files per sample.
Run and library QC report.
Workflow
For raw material: DNA extraction on arrival, included in the kit price. Then incoming quality control of each DNA sample (concentration and purity).
Amplification of the 16S rRNA V3-V4 region (341F/805R) and indexing for sequencing.
Pooling, library QC and sequencing in paired-end 2x300 bp mode.
Demultiplexing and delivery of per-sample FASTQ files.
Ship samples to
Atlas Life Sciences GmbH Probenannahme Friedrichstraße 147 2. OG 10117 Berlin Germany