mRNA Sequencing (30M read-pairs)

Purified total RNA; no extraction required.

• 2.5–1,000 ng of DNA-free total RNA, Qubit-measured
• Dissolved in 25–50 µl of nuclease-free water
• Higher amounts from 200 ng are recommended for quality reasons
• Concentration must not exceed 200 ng/µl. Samples within one project should carry comparable concentrations, for example 20–40 ng/µl or 50–100 ng/µl
• RINe ≥ 7; the RNA must not be degraded
• Purity: OD260/280 1.8–2.1 and OD260/230 2.0–2.2. Lower values indicate contamination such as salts or phenol and can impair library preparation efficiency; such samples are processed at the customer's risk