Small RNA Sequencing

Purified RNA; no extraction required.

• 1 ng–2 µg of DNA-free total RNA, Qubit-measured — or purified small RNA obtained from 1–10 µg of total RNA
• Dissolved in at most 5 µl of nuclease-free water
• RINe ≥ 7. Samples below RINe 7 are processed only after consultation with the NGS laboratory and on your explicit request; no guarantee can be given on the resulting data quality
• Input below 200 ng is processed only after consultation with the NGS laboratory and on your explicit request; no guarantee can be given on the resulting data quality
• Within one experiment the highest input comparable across all samples is used. Samples should carry comparable concentrations, for example 20–40 ng/µl or 50–100 ng/µl
• Purity: OD260/280 1.8–2.1 and OD260/230 2.0–2.2. Lower values indicate contamination such as salts or phenol and can impair library preparation efficiency; such samples are processed at the customer's risk